DNA
Part:BBa_K4593005:Design
Designed by: Huyang Zhang Group: iGEM23_BNDS-China (2023-10-04)
PA#2/8
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added biotin at the 3’ end to test the affinity of the aptamer
Source
In 2016, Regina Stoltenburg and her team discovered PA#2/8 is an aptamer binding the S.aureus protein A.
References
[1] Stoltenburg, R., Krafčiková, P., Víglaský, V., & Strehlitz, B. (2016). G-quadruplex aptamer targeting Protein A and its capability to detect Staphylococcus aureus demonstrated by ELONA. Scientific Reports, 6(1). https://doi.org/10.1038/srep33812